Journal
/
생물학
/
Microinjection of Zebrafish Embryos to Analyze Gene Function
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Microinjection of Zebrafish Embryos to Analyze Gene Function

Microinjection of Zebrafish Embryos to Analyze Gene Function

DOI:
10.3791/1115-v

•

07:18 min

•

March 09, 2009

•

, ,
1Department of Genetics,Harvard Medical School, 2Department of Cardiology,Children’s Hospital Boston

Chapters

  • 00:00Title
  • 00:42Introduction
  • 01:12Zebrafish Egg Collection
  • 02:24Needle Pulling, Loading, and Preparation
  • 04:23Injection
  • 05:48Representative Results: Morpholino and mRNA Injection Phenotypes
  • 06:33Conclusion

Summary

자동 번역

자동 번역

This video shows how morpholino or mRNA can be injected into zebrafish embryos at the one-cell stage to decrease or increase the level of specific gene products during subsequent development.

Tags

MicroinjectionZebrafish EmbryosGene Function AnalysisProtein ManipulationMorpholinosAntisense OligonucleotidesGene ExpressionMRNA InjectionMicroinjection ProcessEmbryo ManipulationInjection Volume

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/1115?article_type=v

Microinjection of A. aegypti Embryos to Obtain Transgenic Mosquitoes
check_url/kr/1115?article_type=v

Microinjection of mRNA and Morpholino Antisense Oligonucleotides in Zebrafish Embryos.
check_url/kr/1115?article_type=v

Generating Chimeric Zebrafish Embryos by Transplantation
check_url/kr/1115?article_type=v

Using the optokinetic response to study visual function of zebrafish
check_url/kr/1115?article_type=v

Microinjection of Medaka Embryos for use as a Model Genetic Organism
check_url/kr/1115?article_type=v

Metabolsk profil Analyse av sebrafisk embryo
check_url/kr/1115?article_type=v

Gene Fangst Bruke Gal4 i sebrafisk
check_url/kr/1115?article_type=v

Force Måling Under Sammentrekning å vurdere muskelfunksjon i sebrafisk Larver
check_url/kr/1115?article_type=v

Høy oppløsning Whole Mount<em> In Situ</em> Hybridisering innenfor sebrafisk embryoer å studere Gene Expression og funksjon
check_url/kr/1115?article_type=v

Analysis of Apoptosis in Zebrafish Embryos by Whole-mount Immunofluorescence to Detect Activated Caspase 3

Read Article