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Chapters
- 00:00Title
- 00:56Introduction
- 01:25‘Inside-Out’ Tissue Preparation
- 04:29Neuronal Bouton Labeling with Fluorescently Labeled Primary Antibody Against HRP
- 05:23Perisynaptic Space Labeling with Dextran Fluorophore Conjugate
- 06:12Mounting the Tissue for Visualization Under a Confocal Microscope
- 07:23Representative Results
- 10:17Conclusion
Wir beschriebenen strukturellen Merkmale der Glia-neuromuskuläre Synapsen in einem Roman Inside-out Gewebe Vorbereitung der Live-Larven unter Verwendung von fluoreszierenden Farbstoffen mit der konfokalen Mikroskopie. Wir gekennzeichnet leben Neuronenendigungen mit fluoreszierenden primären Antikörper gegen HRP und auch visualisiert die perisynaptic Raum mit fluoreszierenden Dextrane.
Tags
VisualizingLiveDrosophilaGlial-neuromuscular JunctionFluorescent DyesGFP Labeled Glial StructuresDeveloping Larval Fly Neuromuscular SynapseLive Glial-nerve-muscle SynapsesOptical PropertiesAccess Of PerfusatesFly LarvaeArtificial HemolymphRhythmic Body ContractionsDissectionPosterior SegmentsBlunt Insect PinEverted Larval Body WallUltra-fine Dissection ScissorsVisceral Side Of Body Wall MusclesGlial Structures At NMJMembrane Targeted GFPGlial Specific PromotersPost-synaptic MembraneSSR (Subsynaptic Reticula)Muscle Expressed Synaptically Targeted DsRedAcutely Label Motor Neuron TerminalsPrimary Antibodies To HRPDye Diffusion PropertiesPerisynaptic Space
