JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Introduction
- 00:17Preparation of dishes
- 00:18Dish for oocyte incubation KSOM
- 01:08Dish for oocyte isolation HCZB and HCZB/hyaluronidase
- 02:23Dish for enucleation and nuclear transfer HCZB/cytocholazin
- 03:30Preparation of pipettes
- 03:31Holding pipette
- 04:53Injection pipette
- 06:30Mouth pipette for oocyte isolation
- 07:07Oocyte isolation
- 09:43Nuclear transfer
- 09:58Enucleation
- 10:31Collection of donor cells
- 11:05Transfer
- 11:50Discussion
