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非放射性原位挪威云杉杂交议定书适用的植物种类范围
JoVE 신문
생물학
Author Produced
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JoVE 신문 생물학
Non-radioactive in situ Hybridization Protocol Applicable for Norway Spruce and a Range of Plant Species
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非放射性原位挪威云杉杂交议定书适用的植物种类范围

DOI:
10.3791/1205-v

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11:56 min

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April 17, 2009

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, , , ,
1Department of Evolutionary Functional Genomics, Evolutionary Biology Center,Uppsala University, 2Department of Plant Biology and Forest Genetics, Uppsala BioCenter,Swedish University of Agricultural Sciences

Chapters

  • 00:00Title
  • 00:45Fixation and Embedding
  • 01:41Sectioning
  • 06:16In Situ Hybridization
  • 11:21Conclusion

Summary

자동 번역

자동 번역

我们描述了一个修改的辛<em>原位</em>杂交协议，它是快速和适用的植物种类广泛，包括挪威云杉。短短几年的调整，包括改变的核糖核酸酶治疗和蛋白酶K的浓度，该协议可用于在不同的组织和物种的研究。

Tags

Non-radioactive In Situ HybridizationProtocolNorway SprucePlant SpeciesExpression Analysis TechnologiesExpression ProfilesIn Situ HybridizationMRNA ExpressionLaboriousTime-consumingOptimizationWoody SpeciesConiferPicea AbiesModified DIG In Situ Hybridization ProtocolRNase TreatmentProteinase K ConcentrationHomologous GenesMale Reproductive OrgansGymnospermAngiosperm SpeciesArabidopsis ThalianaBrassica NapusFloral OrgansMicrosporophylls

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