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Chapters
- 00:00Title
- 00:44Introduction
- 01:33Preparing the needles for microinjection
- 02:18Preparing the embryos
- 03:41Injecting the brain ventricle
- 04:34Imaging
- 05:41Image processing
- 06:37Representative Results/Outcome
- 07:41Conclusion
After neural tube formation, the neuroepithelium constricts and folds while the tube fills with embryonic cerebrospinal fluid (eCSF) to form the embryonic brain ventricles. We developed this ventricle injection technique to better visualize the fluid filled space in contrast to the neuroepithelial shape in a live embryo.
