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Chapters
- 00:10Introduction
- 00:42Expression and Purification of Target Protein and Tagged Ubiquitin
- 03:15Immunoprecipitation
- 04:51SDS-PAGE and Immunostaining
- 05:48Detection of Protein Ubiquitination in vitro
- 07:02Representative Results
- 08:22Conclusion
Tags
Protein UbiquitinationPosttranslational ModificationUbiquitin-activating Enzyme E1Ubiquitin-conjugating Enzyme E2Ubiquitin Ligase E3Deubiquitylating EnzymesProtein StabilityCellular LocalizationBiological ActivityGene MutationsHuman DiseasesCancerNeurodegenerationMetabolic DisordersPathogenesisDetection ProtocolsCultured Cells In VivoTest Tubes In VitroSumolyationNeddylation
