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Chapters
- 00:00Title
- 00:52Introduction
- 01:27Preparing for Dissection
- 02:02Harvesting the E16 Rat Embryos
- 03:19Isolating the Forebrains
- 04:25Digesting and Triturating the Forebrain Tissue
- 06:09Washing, Filtering, and Counting the Dissociated Cells
- 07:03Culturing the Dissociated Cells to Promote Aggregation
- 07:54Preparing the Coverslips and Washing the Aggregates
- 09:15Seeding the Coverslips with the Aggregates
- 11:06Representative Connections Formed Among Aggregates
- 11:29Conclusion
Tags
Rat Brain Aggregate CulturesNeuron Development StudiesGlia Development StudiesIn Vitro SystemCentral Nervous System (CNS)ProgenitorsMature NeuronsGliaAxo-glial InteractionsMultipotent ProgenitorsOligodendroglial Lineage CellsCellular And Molecular LevelSerum-free MediumEmbryonic Rat ForebrainsNeuron-glia InteractionCNS MyelinationIsolate And Grow CNS Aggregate CulturesE16 Rat BrainRegular Dissociated Neuronal Cultures
