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Primary Culture of Adult Rat Heart Myocytes
JoVE 신문
생물학
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JoVE 신문 생물학
Primary Culture of Adult Rat Heart Myocytes
DOI:

11:44 min

June 16, 2009

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Chapters

  • 00:04Title
  • 00:52Introduction
  • 01:18Preparation for Surgery
  • 04:06The Rat Heart Dissection
  • 05:05Myocytes Isolation
  • 10:15Myocyte Cell Culture
  • 10:56Representative Outcome: Cultured Myocytes
  • 11:20Conclusion

Summary

자동 번역

In this paper, we described a typical way to isolate and culture adult rat heart myocytes. Collagenase and protease are used to digest and isolate single myocytes. Myocytes cultured follow this protocol meet most experiment requirements.

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