Journal
/
생물학
/
An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

DOI:
10.3791/1366-v

•

21:16 min

•

July 13, 2009

•

, , , ,
1MatTek Corporation

Chapters

  • 00:05Title
  • 01:32Introduction
  • 02:07The EpiDerm – Reconstructed Human Epidermal Model
  • 04:02Day 0 – Preparing for the Assay
  • 05:21Day 0 – Tissue Conditioning
  • 08:09Day 1 – Chemical Exposure
  • 15:28Day 2 – Media Change
  • 16:03Day 3 – MTT Viability Assay
  • 19:55Representative Results
  • 20:33Conclusion

Summary

자동 번역

자동 번역

In this video, we demonstrate the EpiDerm Skin Irritation test (EpiDerm SIT) developed and validated for in vitro skin irritation testing of chemicals, including cosmetic and pharmaceutical ingredients.

Tags

In Vitro Skin Irritation TestEpiDerm Reconstructed Human Epidermal ModelEpiDerm SITIn Vitro Skin Irritation TestingCosmetic IngredientsPharmaceutical IngredientsGHS Category 2MTT Viability AssayTest ChemicalsPositive ControlNegative Control60-minute Exposure42-hour Post-incubationIncubatorCytokines

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Christopher Hughes: An in vitro model for the Study of Angiogenesis (Interview)

Mouse Epidermal Neural Crest Stem Cell (EPI-NCSC) Cultures

An Allele-specific Gene Expression Assay to Test the Functional Basis of Genetic Associations

Imaging C. elegans Embryos using an Epifluorescent Microscope and Open Source Software

Langsigtet dødelige toksicitet Test med krebsdyr Artemia franciscana</em

Differentiering af Nyfødte musehud afledt stamceller i Germ-lignende celler<em> In vitro</em

Using Caenorhabditis elegans as a Model System to Study Protein Homeostasis in a Multicellular Organism

En In Vitro Model til at studere cellulær Transformation af Kaposis sarkom Herpesvirus

Isolering af mus epidermal keratinocytter og deres in vitro-Clonogen kultur

En Murine Model af en Burn Sår rekonstrueret med en allogeneic hudtransplantation

Read Article