This content is Free Access.
Chapters
- 00:00Title
- 00:55Introduction
- 01:42Preparing Lectin-Conjugated POROS Columns
- 05:15Packing the Lectin-POROS Beads into a PEEK Column
- 08:11HPLC of Samples
- 11:49Desalting the Collected Fractions and PNGase F-digestion
- 14:50Ziptip Desalting of PNGaseF Samples
- 16:34Glycopeptide Analysis Representative Results
- 19:45Conclusion
Lektin-konjugerade POROS pärlor var anställda för HPLC. Glykopeptid standarder varit positiva och negativa kontroller. MARS-14 utarmat var trypsin-rötas humanplasma kromatograferas och genomströmning (FT) och bundna fraktioner samlas in för ESI-LC-MS/MS analyser. Glykopeptider berikades i bundna fraktionen jämfört med FT.
Tags
Lectin HPLC MethodSelectively-glycosylated PeptidesComplex Biological SamplesGlycansPost-translational ModificationsTumor CellsCancer-associated Glycan VariantsBiomarker DevelopmentLectinsSambucus Nigra (SNA)Aleuria Aurantia (AAL)Affinity MatricesPOROS BeadsPEEK ColumnsHigh Pressure Liquid Chromatography (HPLC)Plasma DepletionMultiple Affinity Removal System (MARS-14)Trypsin-digestionFlow-through FractionBound FractionPNGaseFN-linked GlycansLC-MS/MS AnalysisQStar EliteMascot SoftwarePositive ControlsNegative Controls
