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Chapters
- 00:00Title
- 00:55Introduction
- 01:58Two-Dimensional (2-D) Gel Electrophoresis Using Cup-Loading
- 06:30Colloidal Coomassie staining with CBB G-250
- 09:21Representative Results
- 09:47Conclusion
Deze video zal populariseren een colloïdale Coomassie G-250 kleuringsprotocol volgens Kang<em> Et al..</em> Voor de detectie van gemiddeld 4 ng eiwit in gels. De kleuring is binnen twee uur en zonder enige inspanning. We routinematig gebruik van Kang's protocol voor analytische doeleinden in gel op basis van proteomics.
Tags
Coomassie Brilliant Blue G-250Protein StainingSDS-PAGESilver StainingFluorescence StainingMass Spectrometric Protein IdentificationAnalytical Gel-based Proteomic ApproachesSensitivity EnhancementLow-abundant ProteinsColloidal ParticlesMethanolAmmonium SulfatePhosphoric AcidBlue Silver StainingKang Et Al.Aluminum SulfateEthanol
