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Chapters
- 00:00Title
- 00:37Introduction
- 01:18Isolation of Adult Cardiac Myocytes from Rat Heart by Langendorrf Preparation
- 08:02Primary Cell Culture and Adenoviral Transduction
- 09:45Confocal Imaging of 3D Subcellular Structures
- 11:09Imaging of Adult Rat Cardiac Myocytes Representative Results
- 11:43Conclusion
Volwassen hartspiercellen zijn primaire cellen die kunnen worden geïsoleerd uit dierlijke harten en gekweekt voor meerdere dagen. Binnen deze cultuur periode adenovirale gentransfer kan gebruikt worden om genetisch gecodeerd biosensoren (GEBS) of TL-fusie-eiwitten uit te drukken. Beide benaderingen laten cellulaire onderzoeken door middel van confocale microscopie.
Tags
IsolationGenetic ManipulationAdult Cardiac MyocytesConfocal ImagingEmbryonic CellsNeonatal Muscle CellsWorking HeartCardiac Cellular PhysiologyPathophysiologyPharmaceutical InvestigationsTransgenic Animal ModelsLong Term CultureAdenoviral Gene TransferGenetically Encoded BiosensorsFluorescent Fusion ProteinsProtein OverexpressionProtein DownregulationRNAiSubcellular Structure StainingGolgi VisualizationConfocal ImagingZ-stacks ReconstructionCellular Cardiology
