Lentivirus Production

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JoVE 신문
생물학

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JoVE 신문 생물학

Lentivirus Production

DOI:

10.3791/1499-v

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11:42 min

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October 02, 2009

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Xiaoyin Wang, Michael McManus

¹Diabetes Center,University of California, San Francisco – UCSF

Chapters

00:00Title
00:46Introduction
01:15Transfection of 293 Cells To Produce Lentivirus
03:24Lentivirus Harvest
04:39Lentivirus Concentration via Ultracentrifugation
07:24Lentivirus Titration Using Flow Cytometry
11:17Conclusion

Summary

자동 번역

To make lentiviruses, DNA vectors are transfected into human 293 cells. After harvest and concentrating the supernatant, virus titer is determined by fluorescence expression with a flow cytometer.

Lentivirus Production

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