Journal
/
생물학
/
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy
JoVE 신문
생물학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 생물학
Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

Visualizing Single Molecular Complexes In Vivo Using Advanced Fluorescence Microscopy

DOI:
10.3791/1508-v

•

11:26 min

•

September 08, 2009

•

, , ,
1생화학,University of Oxford, 2물리학,University of Oxford

Chapters

  • 00:04Title
  • 01:02Introduction
  • 01:41Preparation of Bacteria
  • 03:49Preparing Cells for Microscopy
  • 07:16Visualizing and Analyzing the Cells
  • 09:29Representative Microscopy Results
  • 10:44Conclusion

Summary

자동 번역

자동 번역

Here we demonstrate the protocols for performing single-molecule fluorescence microscopy on living bacterial cells to enable functional molecular complexes to be detected, tracked and quantified.

Tags

Advanced Fluorescence MicroscopySingle Molecular ComplexesIn Vivo VisualizationLiving CellsCellular MechanismsBiological SystemsSingle-molecule ExperimentationBulk Ensemble-average MeasurementsStochastic EventsHeterogeneous NatureOptical MicroscopyAnalytical Image Analysis ToolsMonitoring ProteinsLiving Bacterial CellMolecular ComplexesFunctioning Biological MachinesPhysiologically Relevant TechniquesMinimally-perturbativeNon-invasiveFluorescently-tagged Protein

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/1508?article_type=v

Visualizing Single-molecule DNA Replication with Fluorescence Microscopy
check_url/kr/1508?article_type=v

Time-lapse Imaging of Mitosis After siRNA Transfection
check_url/kr/1508?article_type=v

Visualizing Bacteria in Nematodes using Fluorescent Microscopy
check_url/kr/1508?article_type=v

Quantitative Analysis of Autophagy using Advanced 3D Fluorescence Microscopy
check_url/kr/1508?article_type=v

Visualizing Cytoplasmic Flow During Single-cell Wound Healing in Stentor coeruleus
check_url/kr/1508?article_type=v

Rapid Analysis and Exploration of Fluorescence Microscopy Images
check_url/kr/1508?article_type=v

Visualizing Stromule Frequency with Fluorescence Microscopy
check_url/kr/1508?article_type=v

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
check_url/kr/1508?article_type=v

Visualizing Lymph Node Structure and Cellular Localization using Ex-Vivo Confocal Microscopy
check_url/kr/1508?article_type=v

Examination of Mitotic and Meiotic Fission Yeast Nuclear Dynamics by Fluorescence Live-cell Microscopy

Read Article