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Chapters
- 01:35Part I: Microprism Optics and Microscope Setup
- 03:25Part II: Animal Surgery and Microprism Insertion
- 07:04Part III: Representative Results and Conclusion
Haakse microprisma ingebracht in de muis neocortex maakt diepe beeldvorming van meerdere corticale lagen met een standpunt typisch gevonden in slice. One-millimeter microprisma bieden een breed veld-of-view (~ 900 um) en ruimtelijke resoluties voldoende om dendritische spines op te lossen. We demonstreren laag V neuronale beeldvorming en neocorticale vasculaire beeldvorming met behulp van microprisma.
Tags
In Vivo ImagingDeep Cortical LayersMicroprismDeep-brain Imaging ProbeReflective CoatingExcitation LightEmission LightSlice PreparationsField-of-viewNon-survival Surgical ProcedureMicroscope SetupLayer V Pyramidal NeuronsThy-1 YFP-H MiceApical DendritesTuftsDendritic SpinesFluorescent DyeBlood VesselsCortexLine-scanningRed Blood Cells (RBCs)CapillariesCellular StructuresCortical Function
