JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:05Title
- 00:54Introduction
- 01:34Preparation of Drosophila
- 04:13Mounting Drosophila Pupae for Imaging
- 05:16Scanning a Tissue of Interest
- 06:18Time-lapse Imaging
- 07:58Three Dimensional Reconstruction of Fluorescence
- 09:59Representative Results/ Outcome
- 10:58Conclusion
Mesoscopic fluorescence tomography operates beyond the penetration limits of tissue-sectioning fluorescence microscopy. The technique is based on multi-projection illumination and a photon transport description. We demonstrate in-vivo whole-body 3D visualization of the morphogenesis of GFP-expressing wing imaginal discs in Drosophila melanogaster.
Tags
Mesoscopic Fluorescence TomographyIn-vivo ImagingDeveloping DrosophilaOptical InterrogationMolecular ChangesFunctional ChangesOptical Imaging MethodsPenetration LimitsOptical MicroscopyDiffusion-imposed LimitsOptical MacroscopyModel OrganismsInsectsAnimal EmbryosSmall Animal ExtremitiesNanometer-resolution Optical ImagingImaging Penetration DepthPhoton ScatteringEntire Embryos ImagingChemical TreatmentScattering ClearancePost-mortem ImagingTwo-photon MicroscopyConfocal Microscopy발생학Drug Discovery
