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Chapters
- 00:00Title
- 00:54Introduction
- 02:09Microdissection of Explants
- 07:16Microdissection and Culture of Central Explants
- 09:18Harvesting the Explants for Protein Analysis
- 10:19Harvesting the Explants for Immunofluorescence
- 12:15Immunofluorescence and Protein Analysis Results
- 15:54Conclusion
Espianti della regione centrale della lente epiteli ratto differenziare in modo sincrono quando coltivate in presenza di FGF-2. Microscopia a immunofluorescenza della presenza di tali culture possono fornisce informazioni romanzo sulla espressione genica e di segnalazione eventi associati a differenziazione terminale.
Tags
PreparationCultureRat Lens Epithelial ExplantsTerminal DifferentiationAnterior SurfaceOcular LensEpithelial CellsProliferationAnnular ZoneCiliary BodyMigrationPosterior ArrayLens Fiber CellsBulk Of The LensIn Vitro InductionFGF-2Neonatal RatsLens CapsuleDissecting TweezersPeripheral RegionsCentral AreaFGF-2 ConcentrationTime CourseSignalingGene ExpressionMolecular TechniquesBiochemical TechniquesPharmacological TechniquesImmunofluorescence Microscopy
