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Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules
JoVE 신문
생물학
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JoVE 신문 생물학
Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

Automated System for Single Molecule Fluorescence Measurements of Surface-immobilized Biomolecules

DOI:
10.3791/1542-v

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10:57 min

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November 02, 2009

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,
1Physics Department,Boston University, 2Department of Biomedical Engineering,Boston University

Chapters

  • 00:00Title
  • 01:44Introduction
  • 02:12Assembling the Microfluidic Cell
  • 05:35Activating the Surface of the Microfluidic Cell for Molecule Immobilization
  • 07:31Preparing the Imaging Buffer and Scanning the Sample
  • 10:03Time-Resolved Single Molecule FRET Traces of DNA Molecules
  • 10:31Conclusion

Summary

자동 번역

자동 번역

In this article we describe how we obtain FRET traces from individual DNA molecules immobilized to a surface using an automated scanning confocal microscope.

Tags

Automated SystemSingle Molecule Fluorescence MeasurementsSurface-immobilized BiomoleculesFluorescence Resonance Energy Transfer MicroscopyFRET MicroscopyNucleic AcidsProteinsSm-FRET MeasurementsTime-tracesBiomolecular DynamicsStatistical AnalysesAutomated Scanning Confocal MicroscopeMicrofluidic CellBuffer ExchangeOxygen ContentConstant TemperatureMicrofluidic Device AssemblyDNA ImmobilizationBuffer PreparationPhotostabilityFluorophoresSetup Preparation

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