This content is Free Access.
Chapters
- 00:07Title
- 01:07Introduction
- 02:18Preparation of Thick Fresh Tissue Sections
- 05:22Staining the Thick Sections with Tetramers
- 10:11Preparing Thin Sections
- 12:17Acquiring Confocal Microscope Images
- 17:56Representative Images of Simultaneous Visualization by ISTH of Immune Effectors and Targets in Tissue Sections
- 19:50Conclusion
En teknik som kombinerar in situ tetramer färgning och in situ hybridisering (ISTH) möjliggör visualisering, kartläggning och analys av rumsliga närhet av virus-specifika CD8 + T-celler till deras virusinfekterade mål och fastställande av kvantitativa relationer mellan dessa immun effectors och mål för infektioner resultat.
Tags
Virus-specific CD8+ T CellsVirus-infected CellsVisualizeIn SituImmune Effector CellsImmune DefensesInfectionSpatial ProximityQuantitative RelationshipsClearanceChronic Persistent InfectionsIn Situ Tetramer StainingIn Situ HybridizationViral-RNA+ CellsBattle Between Immune Defenses And InfectionISTH (in Situ Tetramer Staining And In Situ Hybridization)Mapping Of LocationsE:T RatiosTiming And Magnitude Of The Immune Response
