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L'isolement et la transplantation de cellules souches hématopoïétiques (CSH)
JoVE 신문
생물학
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JoVE 신문 생물학
Isolation and Transplantation of Hematopoietic Stem Cells (HSCs)
DOI:

20:38 min

February 25, 2007

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Chapters

  • 00:00Introduction
  • 00:20Isolating bone marrow cells
  • 00:21Dissecting mouse to isolate bones
  • 01:03Isolating tibia
  • 01:40Isolating femur
  • 02:12Isolating hip bone
  • 02:50Isolating spine
  • 04:07Cleaning bones
  • 06:37Crushing bones
  • 09:33Lineage depletion of bone marrow cells
  • 14:06Antibody staining for FACS
  • 16:06Transplantation into irradiated mouse
  • 18:50Discussion

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