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Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting
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Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

Isolation and Purification of Drosophila Peripheral Neurons by Magnetic Bead Sorting

DOI:
10.3791/1599-v

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12:04 min

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December 01, 2009

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, , ,
1Department of Molecular and Microbiology,George Mason University, 2Krasnow Institute for Advanced Study,George Mason University

Chapters

  • 00:00Title
  • 00:58Introduction
  • 01:31Selecting and Washing Larvae
  • 02:27Dissecting the Larvae and Removing Non-Specific Cells
  • 04:47Dissociating the Tissue into a Single Cell Suspension
  • 08:07Magnetic Bead Cell Sorting
  • 10:05Representative Results of DA Neurons Isolation and RNA Extraction
  • 11:15Conclusion

Summary

자동 번역

자동 번역

In this video-article we present a method for the isolation and purification of Drosophila peripheral neurons using a fast magnetic bead assisted cell sorting strategy. RNA obtained from the isolated cells can be readily used for downstream applications including microarray analyses.

Tags

IsolationPurificationDrosophila Peripheral NeuronsMagnetic Bead SortingPNS NeuronsDendritic Arborization (da) NeuronsNeuronal DevelopmentDendrite MorphogenesisFunctional And Molecular LevelsPure PopulationLow OccurrenceTough Chitinous Larval CuticleSpecific Cell EnrichmentAntibody-coated Magnetic BeadsAge-matched Third Instar LarvaeMouse CD8 Tagged GFP Fusion Protein (UAS-mCD8-GFP)Pickpocket (ppk)-GAL4 DriverPan-da Neuron-specific GAL421-7 DriverLarval CuticleCell Types

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