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Chapters
- 00:00Title
- 00:21Subscription Limit
- 00:42Introduction
- 01:39Incubation of eggs
- 02:56Ex ovo culture
- 06:08Application of substances for ex ovo cam assay
- 07:14Inoculation of cells onto the CAM
- 07:58Intravitreous injection of cells
- 09:18Dissection and grafting of chicken limb buds
- 11:12Preparation and grafting of mouse limb buds
- 14:13Grafting of tumor samples
- 15:36Re-grafting of tumor samples
- 16:53Conclusion
The chick chorioallantoic membrane (CAM) is a unique, naturally immunodeficient supportive culture environment to study angiogenesis and tumorigenesis. This video article demonstrates the different steps in chick ex ovo culture, application of potentially angiogenic substances and successful inoculation of tumor cells and tissues on the surface of the CAM.
