JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 01:40Introduction
- 02:14Pregnant Mouse Laparotomy
- 05:04Gonadal Ridge Dissection
- 08:35Gonadal Ridge Digestion
- 11:03Primordial Germinal Cell Culture
- 12:51Embryonic Germinal Cells
- 13:26Conclusion
Tags
IsolationDerivationMouse Embryonic Germinal CellsPrimordial Germinal CellsDifferentiationCancerInfertilityProtocolDeveloping GonadsMouse EmbryosDissociationMechanical DisruptionCollagenaseMouse Embryo Fibroblast Feeder LayerMitotically InactivatedKnockout MediaLeukemia Inhibitor Factor (LIF)Basic Fibroblast Growth Factor (bFGF)Stem Cell Factor (SCF)PCG IdentificationEstablishment Of Culture ConditionsLong Term CulturesEmbryonic PhenotypePluripotent StateGenetic DamageEpigenetic DamageOxidative Stress
