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Chapters
- 00:00Title
- 00:57Introduction
- 01:16Preparation of the Microfluidic Channels of a BioFlux Plate
- 04:34Preparing the Blood with GPIIb/IIIa Inhibitory Antibody
- 05:41Running the Flow Cell Experiment on the BF1000 Workstation
- 08:19Platelet Adhesion and Aggregation Results
- 08:58Conclusion
The platelet adhesion cascade takes place in the presence of shear flow, a factor not accounted for in conventional (static) well-plate assays. This article reports on a platelet-aggregation assay utilizing a microfluidic well-plate format to emulate physiological shear flow conditions.
Tags
Platelet AdhesionPlatelet AggregationMicrofluidic Flow CellsShear FlowWell-plate AssaysMicrofluidic Well-plate FormatPhysiological Shear FlowExtracellular ProteinsCollagen IVon Willebrand FactorActive PerfusionPneumatic PumpBufferPlatelet InteractionsFluorescent DyeFlow RatesPlatelet ActivationPlatelet Aggregation InhibitorsIC50 Dose Response Data
