JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:40Introduction
- 01:08Preparation of Hippocampal Cultures
- 03:12Immunostaining of Silent Synapses
- 06:56Visualization of Silent Synapses
- 09:20Representative Silent Synapse Data
- 10:21Conclusion
Glutamatergic synapses can switch from an active mode to a silent mode. We demonstrate that presynaptic activity status in dissociated culture of rodent neurons is visualized using a fixable form of the FM1-43 dye to visualize active synapses and immunostaining with vGluT-1 antibody to visualize all glutamate synapses.
Tags
PresynapticSilent SynapsesLight MicroscopySynaptic PlasticityNervous SystemLearnRememberAdaptabilityNeurotoxicPresynaptic PlasticityDigital SwitchingRelease VesiclesGlutamate NeurotransmitterVisualizing Activity StatusDissociated Cell CulturesRodent HippocampusStaining ProfileStyryl Dye FM1-43Synaptic VesiclesImmunostainingVesicular Glutamate Transporter 1 (vGluT-1)Depolarizing StimuliPresynaptic SilencingElectrical SilencingCAMP Signaling Pathways
