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A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo
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A Practical Approach to Genetic Inducible Fate Mapping: A Visual Guide to Mark and Track Cells In Vivo
DOI:

13:36 min

December 30, 2009

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Chapters

  • 00:00Title
  • 00:31Introduction
  • 02:14Administering Tamoxifen by Oral Gavage
  • 04:17Whole Mount Microscopy of E12.5 Embryos
  • 05:10Craniotomy and Adult Whole Mount Microscopy
  • 08:03Explant Preparation and Micro-Dissection of the Ventral Mesencephalon from E12.5 Embryos
  • 09:59Representative Results for Wnt1 Fate-Mapping
  • 12:11Conclusion

Summary

자동 번역

Genetic Inducible Fate Mapping (GIFM) marks and tracks cells with fine spatial and temporal control in vivo and elucidates how cells from a specific genetic lineage contribute to developing and adult tissues. Demonstrated here are the techniques required to fate map E12.5 mouse embryos for epifluorescent and explant analysis.

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