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Live-Imaging van celbeweeglijkheid en actine cytoskelet van individuele neuronen en neurale cellen in de zebravis embryo's
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JoVE 신문 생물학
Live Imaging of Cell Motility and Actin Cytoskeleton of Individual Neurons and Neural Crest Cells in Zebrafish Embryos
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Live-Imaging van celbeweeglijkheid en actine cytoskelet van individuele neuronen en neurale cellen in de zebravis embryo's

DOI:
10.3791/1726-v

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10:52 min

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February 03, 2010

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, , ,
1Genetics Training Program,University of Wisconsin-Madison, 2Department of Anatomy,University of Wisconsin-Madison, 3Department of Zoology,University of Wisconsin-Madison, 4Cell and Molecular Biology Training Program,University of Wisconsin-Madison

Chapters

  • 00:00Title
  • 00:47Introduction
  • 01:18Assembling Injection Slides and Imaging Slides
  • 02:36Injecting DNA Constructs
  • 04:43Mounting Embryos for Imaging
  • 06:524D Imaging of Cell Behaviors on Olympus FV1000 Confocal with IX81 Microscope
  • 08:49Behavior of Individual Labeled Neurons and Neural Crest Cells
  • 10:23Conclusion

Summary

자동 번역

자동 번역

Dit protocol beschrijft beeldvorming van individuele neuronen of neurale cellen in levende zebravis embryo's. Deze methode wordt gebruikt om cellulaire gedrag en actine lokalisatie met behulp van fluorescentie confocal time-lapse microscopie bestuderen.

Tags

Live ImagingCell MotilityActin CytoskeletonIndividual NeuronsNeural Crest CellsZebrafish EmbryosDevelopmentIn VivoOptical ClarityHigh Resolution ImagingCell BehaviorsMigrationAxon OutgrowthGuidanceMechanismsCell LabelingPlasmid DNA InjectionTransient Mosaic Expression PatternProtrusive ActivityMolecular DynamicsTransgenic LinesFine ProtrusionsChanges In Molecular Distribution

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