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Chapters
- 00:00Title
- 00:23Tissue Harvest
- 00:45Collection of Phloem
- 01:07Collection of Secondary Xylem
- 01:26Collection of Reaction Wood: Compression and Opposite Secondary Xylem
- 02:21Liquid Nitrogen Storage
- 02:46RNA Isolation: Tissue Preparation
- 03:20RNA Isolation Day 1
- 04:41RNA Isolation Day 2
- 06:35RNA Precipitation
- 07:16Final Resuspension and Quantification
- 08:02Credits
Many plant tissues, including phloem and xylem from loblolly pine (Pinus taeda L.), contain high levels of phenolics and polysaccharides that interfere with RNA purification. This presentation discusses techniques for the harvest of field-grown tissues and isolation of RNA of sufficient quality for microarrays and other genomic analyses.
