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Chapters
- 00:00Title
- 00:28Introduction
- 01:14Preparation of Aggregates of Transfected COS Cells Using Hanging Drops
- 02:20Preparation of Dorsal Spinal Cord Explants
- 03:31Priming Collagen
- 04:31Dissection of Dorsal Spinal Cord Explants and Positioning with COS Cell Aggregates in Collagen Matrix
- 06:51Visualization of Commissural Axons By Immunohistochemistry
- 08:03Representative Images of Explants
- 08:41Conclusion
This assay assesses the ability of a signaling molecule, here Bone Morphogenetic Protein 7 (BMP7), to reorient commissural axons. An explant of embryonic dorsal spinal cord is cultured adjacent to an aggregate of COS cells secreting the candidate growth factors. Reoriented commissural axons growing within the explant are visualized by immunohistochemistry.
