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A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium
 

A Model of Disturbed Flow-Induced Atherosclerosis in Mouse Carotid Artery by Partial Ligation and a Simple Method of RNA Isolation from Carotid Endothelium

Article DOI: 10.3791/1861-v • 11:01 min • June 22nd, 2010
June 22nd, 2010 •
1, 2, 1, 2, 1, 1, 1, 2, 1,2,3
1Department of Medicine, Division of Cardiology, Emory University, 2Coulter Department of Biomedical Engineering, Georgia Tech and Emory University, 3Department of Bioinspired Science, Ewha Womans University

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요약
Automatic Translation

This describes a partial carotid ligation surgery, which causes disturbed flow conditions and subsequent atherosclerosis development (in two weeks) with intraplaque neo-vascularization (in four weeks) in the mouse common carotid artery. We also describe a novel method of RNA isolation from the carotid intima, providing high purity endothelial RNA.

Tags

Disturbed Flow-induced Atherosclerosis Mouse Carotid Artery Partial Ligation RNA Isolation Endothelium Atherogenesis Low Flow Conditions Oscillatory Flow Common Carotid Artery Direct Evidence Rapid Atherosclerosis Development Apolipoprotein E Knockout Mouse Endothelial Dysfunction Hyperlipidemic Mouse Model Complex Lesion Formation Intraplaque Neovascularization Molecular Mechanisms Flow-dependent Regulation Vascular Biology Therapeutic Interventions
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