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Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast
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Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

Analysis of the Development of a Morphological Phenotype as a Function of Protein Concentration in Budding Yeast

DOI:
10.3791/1863-v

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15:02 min

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March 24, 2010

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, ,
1Department of Biological Sciences and Purdue Center for Cancer Research,Purdue University

Chapters

  • 00:00Title
  • 00:49Introduction
  • 01:30Cell Culture and Protein Induction
  • 04:00Quantification of Cell-Division Phenotype and Cell Death
  • 05:32Visualizing Phenotype-Specific Defects by Fluorescence Microscopy
  • 07:58Determination of Phenotype Development at the Single Cell Level by Time-Lapse Video Microscopy
  • 11:55Effects of ENTH2 Overexpression
  • 14:23Conclusion

Summary

자동 번역

자동 번역

Gene deletion and protein overexpression are common methods for studying functions of proteins. In this article, we describe a protocol for analysis of phenotype development as a function of protein concentration at population and single-cell levels in Saccharomyces cerevisiae.

Tags

Morphological PhenotypeProtein ConcentrationBudding YeastGene DeletionProtein OverexpressionPhenotype DevelopmentPopulation LevelSingle-cell LevelSaccharomyces CerevisiaeEndocytic Adaptor ProteinEpsinDominant Negative ApproachENTH DomainCell Division DefectMislocalization Of Scaffolding ProteinsSeptins

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