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激光显微切割的从细胞的一个子集基因表达分析中的应用果蝇永光盘

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Laser Microdissection Applied to Gene Expression Profiling of Subset of Cells from the Drosophila Wing Disc

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激光显微切割的从细胞的一个子集基因表达分析中的应用果蝇永光盘

DOI:

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15:59 min

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April 30, 2010

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Rosario Vicidomini, Giuseppe Tortoriello, Maria Furia, Gianluca Polese

¹Dipartimento di Biologia Strutturale e Funzionale,University of Naples

Chapters

00:00Title
02:55Genetic Cross
04:55Treatment of Frames
06:12Laser Microdissector Set-Up
07:42Collection of Wing Discs from Drosophila Third instar Transgenic Larvae
09:43Laser MicroDissection
13:21Transcription Profiling
15:27Credits

Summary

자동 번역

激光显微切割技术应用于基因表达谱分析果蝇翼光盘的具体车厢，本地化的RNAi<em>在体内</em>。从沉默和unsilenced车厢相当于地区提取的RNA定量RT - PCR分析，以确定在本土组织微生态的背景下比较的基因表达图谱。

Tags

Laser Microdissection Gene Expression Profiling Subset Of Cells Drosophila Wing Disc Molecular Analysis Cellular Associations In Vivo Interactions Laser-mediated Microdissection Specific Cell Populations Native Context Microscopic Visualization Molecular Integrity Growth Control Cell Differentiation Organogenesis Lineage Restriction Boundaries GAL4-UAS Binary Expression System Transgenic Flies UAS-GFP Transgene

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