JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:47Introduction
- 01:20Preparation of Clarified Tissue Homogenates
- 02:43SDS-PAGE Sample Preparation and Electrophoresis
- 04:12Transferring Proteins from Gels to Membranes
- 05:35Antigen-Epitope Retrieval and Immunoblotting
- 08:13Aβ Multimers by SDS-Page Analysis Using the 6E10 Antibody
- 09:53Conclusion
Se describe una técnica para la preparación de aclarar homogeneizados humanos cortical, la separación de proteínas por SDS-PAGE, la recuperación de antígeno y la inmunotransferencia con un anticuerpo para el péptido Aß. El uso de este protocolo, estamos constantemente detectar Aß monoméricos y multimérica en el tejido cortical de los seres humanos con la patología de Alzheimer.
Tags
SDS-PAGE/ImmunoblotAβ MultimersAntigen-epitope RetrievalAnomalous FoldingPolymerizationβ-amyloid PeptideNeurodegenerative CascadeAlzheimer’s Disease PathogenesisSelf-aggregationAmyloid FibrilsCerebral Senile PlaquesLow Molecular WeightSoluble Aβ MultimersToxic MultimersProteopathiesSDS-polyacrylamide Gel ElectrophoresisImmunoblottingAβ-specific AntibodiesHighly Concentrated Cortical HomogenatesSmall Pore-size Nitrocellulose MembranesClarified Human Cortical Homogenates
