JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
Chapters
- 00:00Title
- 00:47Introduction
- 01:22Producing the GAL4 Enhancer-trap Line
- 02:37Plant Growth and Leaf Protoplasts Isolation
- 07:18Protoplast Sorting by Fluorescence-Activated Cell Sorting (FACS)
- 08:47Leaf Protoplasts Isolation and Sorting by FACS Representative Results
- 09:38Conclusion
The molecular basis of spatial-specific phytochrome responses is being investigated using transgenic plants that exhibit tissue- and organ-specific phytochrome deficiencies. The isolation of specific cells exhibiting induced phytochrome chromophore depletion by Fluorescence-Activated Cell Sorting followed by microarray analyses is being utilized to identify genes involved in spatial-specific phytochrome responses.
