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大蛋白复合物结构质谱分析
JoVE 신문
생물학
This content is Free Access.
JoVE 신문 생물학
Analyzing Large Protein Complexes by Structural Mass Spectrometry
DOI:

15:35 min

June 19, 2010

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Chapters

  • 00:00Title
  • 00:46Introduction
  • 01:18Preparation of Gold-coated Capillaries for Nanoflow Electrospray Ionization
  • 04:12Calibrating the Mass Spectrometer for High Mass Measurements
  • 07:47MS Analysis of Intact Protein Complexes
  • 10:45Tandem Mass Spectrometry: Dissociating Protein Complexes
  • 13:08Representative Results of Large Protein Complexes Analysis by Mass Spectrometry
  • 15:09Conclusion

Summary

자동 번역

质谱法已被证明是一个有价值的工具,用于分析大型蛋白质复合物的。这种方法使多亚基组件组成,化学计量学和整体结构的见解。在这里,我们所描述的,一步一步,如何执行一个结构性的质谱分析,和特性的大分子结构。

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