/

/

Quantification of γH2AX Foci in Response to Ionising Radiation

JoVE 신문
생물학
Author Produced

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 생물학

Quantification of γH2AX Foci in Response to Ionising Radiation

Quantification of γH2AX Foci in Response to Ionising Radiation

DOI:

•

06:53 min

•

April 06, 2010

•

Li-Jeen Mah², Raja S. Vasireddy^2,3, Michelle M. Tang³, George T. Georgiadis, Assam El-Osta³, Tom C. Karagiannis²

¹Epigenomic Medicine, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct, ²Department of Pathology,The University of Melbourne, ³Epigenetics in Human Health and Disease, Baker IDI Heart and Diabetes Institute,The Alfred Medical Research and Education Precinct

Chapters

00:00Title
00:06Introduction
01:25Irradiation
06:01Analysis
06:32Conclusion

Summary

자동 번역

Quantification of DNA double-strand streaks using γH2AX formation as a molecular marker has become an invaluable tool in radiation biology. Here we demonstrate the use of an immunofluorescence assay for quantification of γH2AX foci after exposure of cells to radiation.

Tags

γH2AX Foci Quantification Ionising Radiation DNA Double-strand Breaks H2A Histone Variant Phosphorylation Serine-139 Residue C-terminal SQEY Motif Phosphatidyl-inosito 3-kinase (PIKK) Family Ataxia Telangiectasia Mutated (ATM)DNA-protein Kinase Catalytic Subunit ATM And RAD3-related (ATR)Chromatin Modifications DNA Damage Signalling And Repair Immunofluorescence Microscopy Repair Of DSBs

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Quantitation of γH2AX Foci in Tissue Samples

Evaluation of the Spatial Distribution of γH2AX following Ionizing Radiation

Polarized Translocation of Fluorescent Proteins in Xenopus Ectoderm in Response to Wnt Signaling

To-og tre-dimensjonale Live-Cell Imaging av DNA Damage Response Proteiner

Måling glutation-indusert Fôring Response i Hydra

Undersøkelse av Proteiner Bundet til begynnende DNA i pattedyrceller Bruke BrdU-chip-Slot-vestlig Technique

High Resolution Kvantifisering av krystallinsk cellulose akkumulering i<em> Arabidopsis</em> Roots å overvåke Tissue-spesifikke Cell Wall Modifikasjoner

Metoder for klassifisering av cytoplasmisk foci som mammalstresskorn

Intestinal epitelial regenerering som respons på ioniserende bestråling

Decellulariseringsbasert kvantifisering av skjelettmuskulaturfettinfiltrasjon

Read Article