Journal
/
신경과학
/
Labeling and Imaging Cells in the Zebrafish Hindbrain
JoVE 신문
신경과학
JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다.  전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.
JoVE 신문 신경과학
Labeling and Imaging Cells in the Zebrafish Hindbrain

Labeling and Imaging Cells in the Zebrafish Hindbrain

DOI:
10.3791/1976-v

•

09:17 min

•

July 25, 2010

•

, ,
1Department of Biological Sciences,University of Maryland, Baltimore County, 2Center for Neuroscience,Children’s National Medical Center

Chapters

  • 00:05Title
  • 00:53Introduction
  • 01:27Microinjection in the Zebrafish Hindbrain
  • 03:56Imaging mGFP-labeled Vibratome Sections
  • 06:26Live Imaging
  • 07:49Imaging Cells in the Hindbrain
  • 08:53Conclusion

Summary

자동 번역

자동 번역

Key to understanding the morphogenetic processes that shape the early embryo is the ability to image cells at high resolution. We describe here a technique for labeling single cells or small clusters of cells in whole zebrafish embryos with membrane-targeted Green Fluorescent Protein.

Tags

LabelingImaging CellsZebrafish HindbrainHigh ResolutionPlasmid DNAMosaic ExpressionVisualizationGreen Fluorescent Protein (mGFP)NeurulationConfocal MicroscopeVibratome SectioningAntibody LabelingTime-lapse Confocal MicroscopyNeural Tube FormationCellular BehaviorsHindbrain Region

Article

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

check_url/kr/1976?article_type=v

Time-lapse Live Imaging of Clonally Related Neural Progenitor Cells in the Developing Zebrafish Forebrain
check_url/kr/1976?article_type=v

Live-cell Imaging of Sensory Organ Precursor Cells in Intact Drosophila Pupae
check_url/kr/1976?article_type=v

Electroporation of the Hindbrain to Trace Axonal Trajectories and Synaptic Targets in the Chick Embryo
check_url/kr/1976?article_type=v

Labeling of Single Cells in the Central Nervous System of Drosophila melanogaster
check_url/kr/1976?article_type=v

Retrograde Labeling of Retinal Ganglion Cells in Adult Zebrafish with Fluorescent Dyes
check_url/kr/1976?article_type=v

The Mouse Hindbrain As a Model for Studying Embryonic Neurogenesis
check_url/kr/1976?article_type=v

Ablation of a Neuronal Population Using a Two-photon Laser and Its Assessment Using Calcium Imaging and Behavioral Recording in Zebrafish Larvae
check_url/kr/1976?article_type=v

In Vivo Calcium Imaging of Lateral-line Hair Cells in Larval Zebrafish
check_url/kr/1976?article_type=v

Labeling and Imaging of Amyloid Plaques in Brain Tissue Using the Natural Polyphenol Curcumin
check_url/kr/1976?article_type=v

In vivo Imaging of Fully Active Brain Tissue in Awake Zebrafish Larvae and Juveniles by Skull and Skin Removal

Read Article