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Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

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Multiplex Immunofluorescence Staining and Image Analysis (Video) | JoVE

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Multiplex Immunofluorescence Staining and Image Analysis

Multiplex Immunofluorescence Staining and Image Analysis (Video) | JoVE

Multiplex Immunofluorescence and Spatial Image Analysis of the Tumor Microenvironment

DOI:

10.3791/200119-v

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04:44 min

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June 02, 2023

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Nicolas Huyghe, Elena Benidovskaya, Simon Beyaert, Aurélie Daumerie, Finoula Maestre Osorio, Frank Aboubakar Nana³, Caroline Bouzin, Marc Van den Eynde⁵

¹Institut de Recherche Expérimentale et Clinique (IREC), Pôle MIRO,Université Catholique de Louvain (UCLouvain), ²Institut de Recherche Expérimentale et Clinique (IREC), Pôle de Pneumologie, ORL et Dermatologie (PNEU),Université Catholique de Louvain (UCLouvain), ³Division of Pneumology, Cliniques Universitaires St-Luc,Université Catholique de Louvain (UCLouvain), ⁴IREC Imaging Platform,Université Catholique de Louvain (UCLouvain), ⁵Institut Roi Albert II, Department of Medical Oncology and Gastroenterology,Cliniques Universitaires St-Luc

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Multiplex Immunofluorescence Deparaffinization Endogenous Peroxidase Inhibition Citrate Buffer Microwave Tris-buffered Saline Tween 20 BSA Primary Antibody Poly-HRP Secondary Antibody Tyramide Reagent Pan-Cytokeratin Antibody BisBenzimide Fluorescent Staining Image Analysis

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