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Determining Respiration in Human Sperm Cells by High-Resolution Respirometry

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高分辨率呼吸测定法 - 人类精子的 OXPHOS 分析

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High-Resolution Respirometry – OXPHOS Analysis for Human Spermatozoa

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高分辨率呼吸测定法 - 人类精子的 OXPHOS 分析

Determining Respiration in Human Sperm Cells by High-Resolution Respirometry

DOI:

10.3791/200320-v

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05:03 min

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June 23, 2023

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Pilar Irigoyen, Rossana Sapiro³, Adriana Cassina³

¹Departamento de Histología y Embriología,Unidad Académica Histología y Embriología, ²Departamento de Bioquímica,Unidad Académica Bioquímica, ³Centro de Investigaciones Biomédicas (CEINBIO), Facultad de Medicina,Universidad de la República

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Keywords: High-resolution Respirometry OXPHOS Analysis Human Spermatozoa Respirometer VatLab Oxygen Sensor Calibration Cell Preparation Medium Oxygen Concentration Respiratory Assessment Permeabilized Cell Analysis Digitonin Substrates Complex I Complex II ADP Oligomycin

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