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Isolating Small Extracellular Vesicles and Extracting Their Peptidome

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从分离的小细胞外囊泡（sEV）中提取肽

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Extraction of Peptides from Isolated Small Extracellular Vesicles (sEVs)

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从分离的小细胞外囊泡（sEV）中提取肽

Isolating Small Extracellular Vesicles and Extracting Their Peptidome

DOI:

10.3791/200348-v

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03:04 min

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June 30, 2023

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Jiale Cheng², Jiong Zhu², Yujiao Liu³, Chen Yang³, Yuehui Zhang, Yuchen Liu, Chaozhi Jin, Jian Wang²

¹School of Basic Medical Sciences,Anhui Medical University, ²State Key Laboratory of Proteomics, Beijing Proteome Research Center, National Center for Protein Sciences (Beijing),Beijing Institute of Lifeomics, ³School of Life Sciences,Hebei University

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Keywords: Peptide Extraction Small Extracellular Vesicles SEVs Ultracentrifugation Protease Inhibitor Ultrasonic Crushing Dithiothreitol Iodoacetamide Ultrafiltration Desalting Trifluoroacetic Acid Acetonitrile Mass Spectrometry

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