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Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping
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Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing (Video) | JoVE
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Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing

Poly(A) RNA Purification and Bisulfite Conversion for Next Generation Sequencing (Video) | JoVE

Creating Robust Bisulfite-mRNA Libraries for Transcriptome-Wide Mapping

DOI:
10.3791/200389-v

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05:45 min

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July 07, 2023

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1Institute of Basic Medical Sciences, College of Medicine,National Cheng Kung University, 2Department of Biochemistry and Molecular Biology, College of Medicine,National Cheng Kung University

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Keywords: Poly(A) RNA PurificationBisulfite ConversionNext-generation SequencingTotal RNAOligo(dT) BeadsLysis BufferWash BufferTris-HClRNA ElutionMagnetic SeparationRNA Sample PreparationRNA Enrichment

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