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Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

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用于成像的酵母细胞的制备、成像条件的优化和图像采集

JoVE 신문
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JoVE 신문 생물학

Preparation of Yeast Cells for Imaging, Optimization of Imaging Conditions, and Image Collection

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用于成像的酵母细胞的制备、成像条件的优化和图像采集

Imaging of Mitochondrial Peroxide in Living Yeast Cells Using mtHyper7

DOI:

10.3791/200471-v

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02:33 min

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June 02, 2023

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Emily Jie-Ning Yang, Istvan R. Boldogh², Haojie Ji, Liza Pon², Theresa C. Swayne²

¹Department of Pathology and Cell Biology,Columbia University Irving Medical Center, ²Confocal and Specialized Microscopy Shared Resource in the Herbert Irving Comprehensive Cancer Center,Columbia University Irving Medical Center

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Yeast Cells Mitochondria HyPer7 Microscopy Imaging Fluorescence Confocal Microscopy SCMOS Camera Binning Laser Power Exposure Dynamic Range Noise Level Time-lapse Z-stacks Transmitted Light

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