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Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining

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在μSiM装置中建立血脑屏障单一培养物，用于免疫细胞化学染色和共聚焦分析

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Establishing Blood Brain Barrier Monoculture in µSiM Device for Immunocytochemistry Staining and Confocal Analysis

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在μSiM装置中建立血脑屏障单一培养物，用于免疫细胞化学染色和共聚焦分析

Analysis of Blood-Brain Barrier Models via Immunofluorescence Staining

DOI:

10.3791/200520-v

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05:25 min

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January 12, 2024

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Molly C. McCloskey, Pelin Kasap, Michelle Trempel, Louis P. Widom, Julia Kuebel, Kaihua Chen, Thomas R. Gaborski, Britta Engelhardt, James L. McGrath

¹Department of Biomedical Engineering,University of Rochester, ²Theodor Kocher Institute,University of Bern, ³Department of Biomedical Engineering,Rochester Institute of Technology

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Blood-brain Barrier Monoculture SiM Device Immunocytochemistry Confocal Analysis Cell Culture Collagen Fibronectin HECSR EECM-BMEC Cell Detachment Endothelial Medium Methanol Fixation PBS Washing Blocking Primary Antibody Secondary Antibody Hoechst Staining

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