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면역학 및 감염병학

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Establishment of a 2D Monolayer from 3D Bovine Ileal Enteroid Culture

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从 3D 牛回肠类化合物生成 2D 单层

JoVE 신문
면역학 및 감염병학

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JoVE 신문 면역학 및 감염병학

Generation of 2D Monolayers from 3D Bovine Ileal Enteroids

Please note that all translations are automatically generated. Click here for the English version.

从 3D 牛回肠类化合物生成 2D 单层

Establishment of a 2D Monolayer from 3D Bovine Ileal Enteroid Culture

DOI:

10.3791/200825-v

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04:32 min

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April 05, 2024

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Deborah Molehin, Jack Guinan, Brina Lopez

¹Department of Farm Animal Medicine, College of Veterinary Medicine,Midwestern University

Tags

2D Monolayers 3D Bovine Ileal Enteroids Basement Membrane Extracellular Matrix BME Cell Culture Cell Dissociation Cell Suspension Enteroid Fragments Enteroid Growth Media FBS Wash Media Organoid Growth Media TrypLE Express Enzyme Trypan Blue Dye Exclusion

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