/

/

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

/

Isolement et dissociation de l’intestin des larves de poisson-zèbre pour l’enrichissement des cellules intestinales par FACS

JoVE 신문
발생학

JoVE 비디오를 활용하시려면 도서관을 통한 기관 구독이 필요합니다. 전체 비디오를 보시려면 로그인하거나 무료 트라이얼을 시작하세요.

JoVE 신문 발생학

Isolation and Dissociation of Gut from Zebrafish Larvae for FACS Enrichment of Gut Cells

Please note that all translations are automatically generated. Click here for the English version.

Isolement et dissociation de l’intestin des larves de poisson-zèbre pour l’enrichissement des cellules intestinales par FACS

Processing Gut Cells from Zebrafish Larvae for Single-Cell RNA Sequencing

DOI:

10.3791/200860-v

•

02:37 min

•

November 10, 2023

•

Naomi J. M. Kakiailatu, Laura E. Kuil², Eric Bindels, Joke T. M. Zink, Michael Vermeulen, Veerle Melotte, Maria M. Alves

¹Department of Clinical Genetics,Erasmus University Medical Center – Sophia Children’s Hospital, ²Division of Psychosocial Research and Epidemiology,the Netherlands Cancer Institute, ³Department of Hematology,Erasmus Medical Center, ⁴Department of Pathology, GROW-School for Oncology and Developmental Biology,Maastricht University Medical Center

Tags

Gut Zebrafish Larvae FACS Cell Isolation Dissociation Papain Agarose Plate HEPES E3 Medium Dissection Intestine Cell Strainer Centrifugation DAPI Cell Labeling

Embed

플레이리스트에 추가

Usage Statistics

Related Videos

Isolement intestinal de larves de poisson-zèbre pour le séquençage de l’ARN unicellulaire

Obtention de larves de poisson-zèbre de type sauvage et transgénique et de dissociation de larves entières de type sauvage

Isolement et dissociation de l’intestin des larves de poisson-zèbre pour l’enrichissement des cellules intestinales par FACS

Enrichissement FACS de la suspension de cellules intestinales de poisson-zèbre pour scRNAseq

Read Article