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Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation
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Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies
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Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies
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Eluting Epitope Tag Precipitated Proteins and Immunoblotting for Protein-Protein Interaction Studies

Protein Interaction Analysis in HEK-293 Cells Using Co-Immunoprecipitation

DOI:
10.3791/201505-v

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02:58 min

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January 05, 2024

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, , , , , , , , ,
1Department of Molecular and Internal Medicine, Graduate School of Biomedical & Health Sciences,Hiroshima University

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Keywords: Epitope TagProtein PrecipitationImmunoblottingProtein-protein InteractionCentrifugationProtein Lysis BufferProtease InhibitorSample BufferSDS-PAGEPVDF MembranePrimary AntibodyDYKDDDDK-PRDM16HA-EHMT1HEK 293 Cells

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