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Chapters
- 00:00Title
- 00:41Introduction
- 01:20Preparation of Essential Proteins for Nuclear Transport Experiment
- 06:10Preparation of the Cell System for Single Molecule Experiment
- 08:52Single Molecule Microscopy
- 10:22Representative Single Molecule Imaging
- 11:39Conclusion
Tags
Single-molecule ImagingNuclear TransportSingle Molecule Fluorescence MicroscopyBiological ReactionsMolecular InteractionsHigh Temporal ResolutionHigh Spatial ResolutionNarrow-field Epifluorescence MicroscopyNucleocytoplasmic TransportSingle Molecule ApproachKineticsNuclear Transport TimeEfficiencySignal-dependent Cargo MoleculesSignal-independent Cargo MoleculesImproved Spatiotemporal ResolutionTransient Active TransportPassive Diffusion EventsNuclear Pore Complexes (NPC)Semi-intact CellsBinding EventsTrafficking Events
