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Chapters
- 00:00Title
- 01:05Introduction
- 01:40Spheroplasting and Osmotic Lysis
- 05:48Resuspending the Membrane Pellet
- 07:58Sucrose Density Gradient Centrifugation
- 09:51Representative Results of Outer Membrane Isolation from F. tularensis
- 10:24Conclusion
Tags
IsolationFrancisella TularensisOuter MembranesGram-negativeCoccobacillusZoonotic DiseaseTularemiaInfectious DoseVirulence FactorsOuter Membrane Proteins (OMPs)Host Cell BindingEntryIntracellular SurvivalVirulenceImmune EvasionProtective VaccinesGram-negative BacteriaBulk Membrane Extraction TechniquesSonicationCentrifugationDetergent ExtractionPeriplasmic ContaminantsCytoplasmic ContaminantsInner Membrane (IM)SpheroplastingOsmotic LysisSucrose Density Gradient CentrifugationLipopolys
