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胞外多糖的OLIGO质谱分析（OLIMP）
JoVE 신문
생물학
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JoVE 신문 생물학
OLIgo Mass Profiling (OLIMP) of Extracellular Polysaccharides
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胞外多糖的OLIGO质谱分析（OLIMP）

DOI:
10.3791/2046-v

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08:43 min

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June 20, 2010

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, ,
1Energy Biosciences Institute,University of California, Berkeley, 2Department of Plant and Microbial Biology,University of California, Berkeley

Chapters

  • 00:00Title
  • 00:45Introduction
  • 01:23Solubilisation of Polymers from Isolated Cell Walls
  • 03:50MALDI-TOF MS Analysis of the Released Oligosaccharides
  • 05:42In situ OLIMP Analysis
  • 07:19OLIMP Representative Results and Analysis
  • 08:18Conclusion

Summary

자동 번역

자동 번역

描述一个快速的方法是争取到在细胞外基质多糖结构的见解。该方法采用glycosylhydrolases特异性，灵敏度和质量允许微量的材料加以分析法的优势。这种技术直接用于组织本身的适应性。

Tags

OLIgo Mass ProfilingExtracellular PolysaccharidesGlycoproteinsProteoglycansPolysaccharidesExtracellular MatrixGlycostructuresCell-to-cell CommunicationCell-to-environment CommunicationSugar MoietiesPlant Cell WallsBiopolymersCarbon-neutral Renewable ResourcePetrochemicalsFossil FuelFuel ConversionEnzymatic DegradationChemical DegradationGlycostructures AnalysisOLIgo Mass Profiling Method

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