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Chromatine Immunoprecipitatie (ChIP) om Assay Dynamic histon-eiwitten in Actieve genexpressie in menselijke cellen
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JoVE 신문 생물학
Chromatin Immunoprecipitation (ChIP) to Assay Dynamic Histone Modification in Activated Gene Expression in Human Cells
DOI:

13:20 min

July 29, 2010

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Chapters

  • 00:05Title
  • 01:11Introduction
  • 01:33Crosslinking
  • 03:32Preparing Chromatin
  • 05:37Chromatin IP (ChIP)
  • 07:50Collecting Immunocomplexes
  • 10:01Recovering the DNA
  • 11:45Representative Results
  • 12:44Conclusion

Summary

자동 번역

Dit protocol beschrijft hoe chromatine immunoprecipitatie (ChIP) wordt gebruikt om de dynamische wijzigingen studie naar de chromatine sjabloon die transcriptie veroorzaakt door een signaal transductie route te reguleren.

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